Analysis Note

ControlHEK293 cell lysate

Application

Anti-beta Arrestin 1 Antibody, clone 3H11.2 detects level of beta Arrestin 1 & has been published & validated for use in WB & IF.

Research Sub CategorySensory & PNSG-proteins

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 (beta Arrestin 1), Arrestin 3 (beta Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, beta Arrestins 1 and 2 are ubiquitously expressed. Beta Arrestins appear to act as negative regulators on G protein-coupled receptors, (Lefkowitz, 1998). Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases, (Lin, 1998; Miller, 2001).

Immunogen

KLH conjugated synthetic peptide corresponding to amino acids 357-372 of human beta arrestin 1.

Epitope: a.a. 357-372

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ammonium Sulfate Precipitation

Format: Purified

Purified in PBS with 0.05% NaN3.

Quality

Evaluated on a representative lot by Western blot in HEK293 cell lysate.

Specificity

Other species have not been tested.

Reacts with human beta-arrestin 1.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Target description

~47 kDa